Vaccine Production

Day 1

Lecture:

  • Basic Virology and Immunology
  • Fundamentals of Cell Culture
  • Anchorage dependent and suspension cell lines: Cell line characterization for production of biologics

Laboratory:

  • Aseptic Techniques and Biosafety
  • Basic Cell Culture techniques:
    1. Anchorage dependent cells
    2. Suspension cells
    3. Monolayer trypsinization
    4. Cell counting
    5. Viability measurement
    6. Sub-culturing cells
    7. Group Discussions

Day 2

Lecture:

  • Influenza Vaccine Production – Overview
  • Scale-Up strategies for mammalian cell systems
  • Stirred-tank bioreactors

Laboratory:

  • Infection of Cultures with virus
  • SRID Set-up
  • Bench-top Bioreactor set-up
  • Group Discussions

Day 3

Lecture:

  • Microcarrier cell culture for production of vaccines
  • Hollow-Fiber cell culture technology
  • Purification Strategies: Centrifugation

Laboratory:

  • Microcarrier cell culture Set-up in disposable
  • Hemagglutination Assay
  • SRID Assay
  • Group Discussions

Day 4

Lecture:

  • Critical Issues in vaccine production
  • Quality control issues
  • Purification Strategies: Filtration and Chromatography

Laboratory:

  • Hollow-Fiber bioreactor
  • SRID Data Analysis
  • Group Discussions

Day 5

Lecture:

  • Alternative Technologies for production of vaccines:
    1. Insect Cell Systems
    2. Microbial systems
    3. Single use bioreactor systems
  • cGMP and Validation issues: An overview
  • Final Course evaluation and distribution of certificates

Adjourn at noon

This is a week long course designed to benefit those who are new to vaccine manufacturing in cell-based systems and those who want to add to their knowledge of vaccine production. Lectures will emphasize the principles of vaccinology, virology cell culture, scale-up strategies, purification of vaccines and regulatory issues related to vaccine manufacturing in a GMP environment. Hands-on laboratory work includes cell culture, viability studies, viral infection, vaccine production in bioreactors systems, purification and downstream processing of vaccines and safety protocols.

 

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Instructors:

  • Kamal Rashid, Ph.D., Center for Integrated BioSystems, USU
  • Bart Tarbet, Ph.D., Institute for Antiviral Research, USU
  • Brett Adams, MS., Institute for Antiviral Research, USU
  • Shaun Barnett, Bioprocess Engineer, Center for Integrated BioSystems, USU
  • Ma Sha, Ph.D., Eppendorf/ New Brunswick
  • Corey Card, MS., Thermo Fisher Scientific Inc.