Day 1

Lecture:

• Introduction to downstream processing
• Principles of centrifugation
• Cell disruption and protein capture

Laboratory:

• Cell harvest from fermentor
• Cell disruption and clarification

Day 2

Lecture:

• Principles of chromatography
• Ion exchange, hydrophobic interaction
• Affinity chromatography, gel filtration

Laboratory:

• Protein chromatography

Day 3

Lecture:

• Chromatography: Purification strategies Optimization
• Filtration:
  1. Filtration principles-conventional and tangential flow
  2. Filtration techniques-microfiltration, ultrafiltration, and diafiltration

Laboratory:

• Tangential flow filtration
• Protein polishing using gel filtration chromatography

Day 4

Lecture:

• MS technologies in high throughput proteomics
• Protein identification utilizing MALDI-QTOF mass spectrometry

Laboratory:

• Protein characterization using MALDI-QTOF mass spectrometry

Adjourn at noon