Day 1

Lecture:

• Fundamentals of cell culture, primary culture, cell transformation
• Media design, serum, serum-free media

Laboratory:

• Laboratory safety, aseptic techniques, culturing and subculturing, cell viability, growth parameters and culture expansion for seeding reactors

Day 2

Lecture:

• Quality control of cell culture laboratory
• Mycoplasma detection and control
• Contamination control

Laboratory:

• Chromosome Analysis
• Growth parameters and growth curve experiments
• Hollow-fi ber bioreactor demonstrations
• Microcarrier cell culture preparations

Day 3

Lecture:

• Microcarrier and hollow fi ber cell culture
• Stirred-tank bioreactor: design and operations

Laboratory:

• Bench-top reactor setup and preparation for sterilization
• Media handling and asepsis, pH and DO probe preparation and calibration
• Seeding the reactors

Day 4

Lecture:

• Cell line characterization for production of biologics
• Cell Culture – single use bioreactor systems

Laboratory:

• Follow-up on the stirred-tank reactor experiments
• Demonstration of single-use bioreactors

Adjourn at noon