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Biography

Daryll B. DeWald

 

  • Daryll B. DeWald , Ph.D.
  • Associate Director (Research & Recruiting), CIB
  • 4700 Old Main Hill
  • Logan, UT 84322-4700
  • Phone: (435) 760-3685
  • Fax: (435) 797-2766

 

Overview

As Associate Director of Research at the Center for Integrated BioSystems, Dr. Daryll DeWald?s primary role is oversight of research that takes place under the CIB roof, whether USU-related or with other off-site collaborators. In addition to research responsibilities, Daryll manages the CIB?s educational outreach programs to Utah state high school students and their teachers, an effort to attract top scientific seniors to Utah State University. He is also involved in managing the CIB undergraduate research program.

Dr. DeWald is a biochemist and has active research programs or projects in plant functional genomics, plant cell signaling, and mammalian cell signaling. His work has explored the role of lipids that control cellular communication during plant stress acclimation and cellular protein trafficking, and how lipids regulate cancer cell metastasis. He has co-authored more than 40 peer-reviewed publications and has managed over $3,000,000 in research funding. During the last decade he has been advisor to more than 10 graduate students and 40 undergraduate research students. His instructional emphases have been coursework in Cell Biology, Advanced Cell Biology, and Plant Physiology.

Academic & Industrial Experience
Associate Director Center for Integrated BioSystems
Utah State University		 2002–present
Associate Professor Biology Department
Utah State University		 2001–present
Adjunct Assoc. Professor Biological Irrigation Engineering
Utah State University		 2004–present
Assistant Professor Biology Department
Utah State University		 1995–2001
Postdoctoral Fellow H.H.M.I.
UC San Diego		 1993–1995
Ph.D. Graduate Student Biochemistry Department
Texas A&M University		 1988–1993
Biochemist Biopolymer Chemistry
The Upjohn Company		 1983–1988
Awards & Honors
NIH ? Mathematical Modeling Gene Expression Metabolism Study Section 2005
American Heart Association, Undergraduate Research Roundtable, Moderator 2004–2005
American Heart Association, Fellowship and Grant Study Sections 2004–present
USU College of Science, Undergraduate Research Mentor of the Year 2004
NIH ? NIGMS MBRS Study Section 2002
NIH ? NIEHS Study Section 2002
Phi Kappa Phi Honor Society 2000
N.S.F. Postdoctoral Fellow in Plant Biology 1993–1996
Phi Lambda Upsilon Honorary Chemical Society, Beta Beta Chapter 1983
Gamma Sigma Delta Agriculture Honor Society 1983
Education
Texas A&M University Ph.D., Biochemistry Department 1993
University of Wyoming B. S., Biochemistry Department 1983
Research Focus
  • Signal transduction pathways with an emphasis on phosphoinositides
  • Plant stress acclimation
  • Cellular signaling during plant stress
  • Phosphoinositide signaling during breast cancer metastasis
  • Regulation of intracellular trafficking
Patents
Publications

Strahl, T., Hama, H., DeWald, D.B., Thorner, J. (2005) Pik1 PtdIns 4-kinase regulates protein secretion at the Golgi, submitted, revised resubmission, J. Cell Biol.

DeWald, D.B., Ozaki, S., Malaviya, S., Shope, J.C., Manabe, K., Prestwich, G.D. (2005) Cellular calcium mobilization in response to phosphoinositide delivery, Cell Calcium 38:59-72

Williams, M., Torabinejad, J., Parker, K., Cohick, E., Hortter, M., Drake, E.S., Thompson, J.E., and DeWald, D.B. (2005) Mutations in the Arabidopsis phosphoinositide phosphatase gene SAC9 lead to overaccumulation of PtdIns(4,5)P2 and constitutive expression of the cold-response pathway, Plant Physiol., 138:686-700.

DeWald, D.B., Torabinejad, J., Samant, R.S., Johnston, D., Erin, N., Shope, J.C., Xie, Y., and Welch, D.R. (2005) Metastasis suppression by BRMS1 involves reduction of phosphoinositide signaling in MDA-MB-435 breast carcinoma cells, Cancer Res., 65:713-717.

Hama, H., Torabinejad, J., Prestwich, G.D., and DeWald, D.B. (2004) Measurement and Immunofluorescence of Cellular Phosphoinositides, in Methods in Molecular Biology, 248:243-258.

Yu, J.W., Mendrola, J.M., Audhya, A., Singh, S., Keleti, D., DeWald, D.B., Murray, D., Emr, S.D., and Lemmon, M.A. (2004) Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains, Mol. Cell, 13:677-88.

Shope, J.C., DeWald, D.B., and Mott, K.A. (2003) Changes in surface area of intact guard cells are correlated with membrane internalization, Plant Physiol., 133:1314-21.

Shaw, J.D., Hama, H., Sohrabi, F., DeWald, D.B., and Wendland, B. (2003) PtdIns(3,5)P2 is Required for Delivery of Endocytic Cargo Into the Multivesicular Body. Traffic, 4:479-490.

DeWald, D.B. (2003) Analyses of PtdIns(3,4,5)P3 in 3T3 L1-preadipocytes. in Methods in Molecular Medicine, 83:145-154.

Ha, S., Torabinejad, J., DeWald, D.B., Wenk, M.R., Lucast, L., DeCamilli, P.,Newitt,R.A., Aebersold, R., and Nothwehr, S.F. (2003) The synaptojanin-like protein Inp53 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway. Mol. Biol. Cell, 14:1319-1333.

DeWald, D.B. (2002) Phosphoinositide signaling: vac to the future in fab1 kinase regulation. Curr Biol., 12: R491-2.

Feng, L., Manabe, K., Shope, J.C., Widmer, S., DeWald, D.B., and Prestwich, G.D. (2002) A real-time fluorogenic phospholipase A2 assay for biochemical and cellular activity measurements. Chem. & Biol., 9(7):795-803.

Prestwich, G.D., Chen, R., Feng, L., Ozaki, S., Ferguson, C.G., Drees, B.E., Neklason, D.A., Porter-Gill, P., Kang, V.H., Shope, J.C., Neilsen, P.O., and DeWald, D.B. (2002) In situ Detection of Phospholipid and Phosphoinositide Metabolism, Advances in Enzyme Regulation, 42:19-38.

Chen R., Kang, V.H., Chen, J., Shope, J.C., Torabinejad, J., DeWald, D.B., and Prestwich, G.D. (2002) A Monoclonal Antibody to Visualize PtdIns(3,4,5)P3 in Cells. J. Histochem. Cytochem. 50:697-708.

Budovskaya, Y.V., Hama, H., DeWald, D.B. and Herman, P.K. (2002) The C-terminus of the Vps34p PI 3-kinase is necessary and sufficient for the interaction with the Vps15p protein kinase. J. Biol. Chem., 277:287-94.

Ha, S., Bunch, J.T., Hama, H., DeWald, D.B. and Nothwehr, S.F. (2001) A novel mechanism for localizing membrane proteins to the yeast trans-Golgi network requires function of a synaptojanin-like protein. Mol. Biol. Cell, 12:3175-3190.

DeWald, D.B., Torabinejad, J., Jones, C., Shope, J., Cangelosi, A., Thompson, J., Prestwich, G.D and Hama, H. (2001) Rapid accumulation of phosphatidylinositol 4,5-bisphosphate and inositol 1,4,5-trisphosphate correlates with calcium mobilization in Salt-Stressed Arabidopsis thaliana. Plant Physiol,126:759-769.

Monks, D.E., Aghoram, K., Courtney, P.D., DeWald, D.B., and Dewey, R.E. (2001) Hyperosmotic stress induces the rapid phosphorylation of a soybean phosphatidylinositol transfer protein homolog through activation of the protein kinases SPK1 and 2, The Plant Cell, 13:1205-1219.

Stratford, S., DeWald, D.B., and Summers, S. (2001) Ceramide dissociates 3?-phosphoinositide production from pleckstrin homology domain translocation Biochem J. 354, 359-368.

Ozaki, S., DeWald, D.B., Shope, J.C., Chen, J., and Prestwich, G.D. (2000) Delivery of phosphoinositides and inositol polyphosphates into cells using polyamine carriers. Proc. Natl. Acad. Sci. 97:11286-11291.

Hama, H., Takemoto, J.Y., and DeWald, D.B. (2000) Analysis of phosphoinositides in protein trafficking. in Methods: A Companion to Methods in Enzymology. 20, 465-473.

Hama, H., Schneiders, E.A., Thorner, J., Takemoto, J.Y., and DeWald, D.B. (1999) Direct Involvement of phosphatidylinositol 4-phosphate in secretion in the yeast Saccharomyces cerevisiae. J. Biol. Chem, 274: 294-300.

Tall, G.G., Hama, H., DeWald, D.B., and Horazdovsky, B.F. (1999) The phosphatidylinositol 3-phophosphate binding protein Vac1 interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting. Mol. Biol. Cell 10:1873-1889.

Stock, S., Hama, H., DeWald, D.B., and Takemoto, J.Y. (1999) SEC14-dependent secretion in Saccharomyces cerevisiae: non-dependence on sphingolipid synthesis-coupled diacylglycerol production. J. Biol. Chem. 274:12979-12983.

Forney, J.R., DeWald, D.B., Yang, S., Speer, C.A., and Healey, M.C. (1999) A Role for Phosphatidylinositol 3-Kinase and Cytoskeleton Remodeling During Cryptosporidium Parvum Infection. Infec. Immun. 67:844-852.

DeWald, D.B., Wurmser, A.E., and Emr, S.D. (1997). Regulation of Saccharomyces cerevisiae Vps34p phosphatidylinositol 3-kinase. Biochem. Soc. Trans. 25:1141-1146.

Stack, J.H., Herman, P.K., DeWald, D.B., Marcusson, E.G., Cereghino, J.L., Horazdovsky, B.F., and Emr, S.D. (1995) Novel protein kinase/phosphatidylinositol 3-kinase complex essential for receptor-mediated protein sorting to the vacuole in yeast. Cold Spring Harbor Symposium on Quantitative Biology, vol. LX, 157-170.

*Takegawa, K., *DeWald, D.B., and Emr, S.D. (1995). Phosphatidylinositol-specific PI 3-kinase from Schizosaccharomyces pombe, spvps34p, required for normal cell growth and vacuole maintenance. J. Cell Sci. 108:3745-3756.

Horazdovsky, B.F., DeWald, D.B., and Emr, S.D. Protein Transport to the Yeast Vacuole (1995) Current Opinion in Cell Biology, 7:544-551.

Brown, W.J., DeWald, D.B., Emr, S.D., Plutner, H., and Balch, W.E. Role for Phosphatidylinositol 3-Kinase in the Sorting and Transport of Newly Synthesized Lysosomal Enzymes in Mammalian Cells (1995) J. Cell Biology, 130:781-796.

Yamamoto, A., DeWald, D.B., Boronenkov, I.V., Anderson, R.A, Emr, S.D., and Koshland, D. A . Novel PI(4)P 5-Kinase Homolog, Fab1p, Essential for Normal Vacuole Function and Morphology in Yeast (1995) Mol. Biol. Cell 6:525-539.

Stack, J.S.*, DeWald, D.B.*, Takegawa, K., and Emr, S.D. Vesicle-Mediated Protein Transport: Regulatory Interactions Between the Vps15 Protein Kinase and the Vps34 PtdIns 3-kinase Essential for Protein Sorting to the Vacuole in Yeast (1995) J. Cell Biol. 129:321-334.

Sadka, A., DeWald, D.B., May, G.D., Park, W.D., and Mullet, J.E. Phosphate Modulates Transcription of Soybean VspB and Other Sugar-Inducible Genes (1994) Plant Cell, 6:737-749.

DeWald, D.B., Sadka, A., and Mullet, J.E. Sucrose Modulation of Soybean Vsp Gene Expression is Inhibited by Auxin (1994) Plant Physiol. 104:439-444.

Mason, H.S., DeWald, D.B., and Mullet, J.E. Identification of a Methyl Jasmonate-Responsive Domain in the Soybean vspB Promoter (1993) Plant Cell 5:241-251.

DeWald, D.B., Mason, H.S.,and Mullet, J.E. The Soybean Vegetative Storage Proteins VSP and VSP are Acid Phosphatases Active on Polyphosphates (1992) J. Biol. Chem. 267:15958-15964.

Mason, H.S., DeWald, D.B., Creelman, R.A., and Mullet, J.E. Co-regulation of Soybean Vegetative Storage Protein Gene Expression by Methyl Jasmonate and Soluble Sugars (1992) Plant Physiol. 98:859-867.

Pearson, J.D., DeWald, D.B., Mathews, W.R., Mozier, N.M., Zurcher-Neely, H.A., Heinrikson, R.L., Morris, M.A., McCubbin, W.D., McDonald, J.R. Fraser, E.D., Vogel, H.J., Kay, C.M., and Walsh, M.P. Amino Acid Sequence and Characterization of a Protein Inhibitor of Protein Kinase C (1990) J. Biol. Chem. 265:4583-4591.

DeWald, D.B. and Pearson J.D. Isolation and Sequence Analysis of Proteins from Mouse Forebrain Using Two-Dimensional Gel Electrophoresis Coupled to High-Pressure Liquid Extrusion (1989) Anal. Biochem. 180:340-348.

DeWald, D.B., Colca, J.R., McDonald, J.M., and Pearson, J.D. Separation of Tyrosine Phosphorylated Calmodulin from Calmodulin Using Non-Porous Anion-Exchange HPLC (1988) J. Liq. Chromatography 11:2109-2120.

Laurino, J.P., Colca, J.R., Pearson, J.D., DeWald, D.B., and McDonald, J.M. The In Vitro Phosphorylation of Calmodulin by the Insulin Receptor Tyrosine Kinase (1988) Arch. Biochem. Biophys. 265:8-21.

Pearson, J.D., DeWald, D.B., and Colca, J.R. Separation, Isolation and Sequencing of Polypeptides from Rodent Pituitary and Whole Brain by Two-Dimensional Gel Electrophoresis Coupled with Pressure Extraction, in Protein Purification: Micro to Macro (1987) p. 27-34 (Editor, R. Burgess) Alan R. Liss Inc.

Pearson, J.D., McCroskey, M.C., and DeWald, D.B. Separation of Protein Hormones (1987) J. Chromatography 418:245-276.

Colca, J.R., DeWald, D.B., Pearson, J.D., Palazuk, B.J., Laurino, J.P., and McDonald, J.M. Insulin Stimulates the Phosphorylation of Calmodulin in Intact Adipocytes (1987) J. Biol. Chem. 262:11399-11402.

DeWald, D.B., Adams, L.D., and Pearson, J.D. Two-Dimensional Gel Electrophoresis of Polypeptides in Proteins: Structure and Function (1987) p. 133-138 (Editor, J. L'Italien), Plenum Press.

Pearson, J.D., DeWald, D.B., Zurcher-Neely, H.A., Heinrikson, R.L., and Poorman, R. Sequencing Proteins from Two-Dimensional Gels Using a Liquid Pressure Extraction Technique in Methods in Proteins Sequence Analysis (1986) p. 295-302 (Editor, K. A. Walsh) Humana Press.

DeWald, D.B., Adams, L.D., and Pearson, J.D. A Non-Urea Electrophoretic Gel System for Resolution of Polypeptides of Mr 2,000 to Mr 200,000 (1986) Anal. Biochem. 154:502-508.

DeWald, D.B. and Lewis, R.V. Enkephalin Containing Polypeptide Levels in Normaltensive and SHR Rat Adrenal Glands (1983) Peptides, 4:121-123.

Lewis, R.V. and DeWald, D. Reverse-Phase HPLC of Proteins: Effects of Various Bonded Phases (1982) J. Liq. Chromatography 5:1367-1374.

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