Courses
Downstream Processing of Biotechnology Products
In-depth presentations of the theoretical aspects of downstream processing are combined with unified hands-on laboratory sessions where the participant completes the steps to create a finished product from whole cells in culture.
Day 1
Lecture:- Introduction to downstream processing
- Principles of centrifugation
- Cell disruption and protein capture
- Cell harvest from fermentor
- Cell disruption and clarification
Day 2
Lecture:- Principles of chromatography
- Ion exchange, hydrophobic interaction
- Affinity chromatography, gel filtration
- Protein chromatography
Day 3
Lecture:- Chromatography: Purification strategies Optimization
- Filtration: Filtration principles-conventional and tangential flow
Filtration techniques-microfiltration, ultrafiltration, and diafiltration
- Tangential flow filtration
- Protein polishing using gel filtration chromatography
Day 4
Lecture:- MS technologies in high throughput proteomics
- Protein identification utilizing MALDI-QTOF mass spectrometry
- Protein characterization using MALDI-QTOF mass spectrometry
Instructors:
- Peter Moore, Ph.D., GE Healthcare, USA
- John Peltier, Ph.D., Prolexys Pharmaceutical, Inc. USA
- Kamal Rashid, Ph.D., Center for Integrated BioSystems, USU
- Tim Taylor, Ph.D., Biological and Irrigation Engineering, USU