Courses
Techniques In Animal Cell Culture & Scale-Up Strategies
This course teaches the latest techniques used with mammalian cell culture. Laboratory work includes hands-on experience in cell growth and subculturing, and extensive work with different types of bench scale bioreactors. Also included are lectures and laboratory work on cloning, nuclear transfer, and stem cell manipulations.
Day 1
Lecture:- Fundamentals of cell culture, primary culture, cell transformation
- Media design, serum, serum-free media
- Laboratory safety, aseptic techniques, culturing and subculturing, cell viability, growth parameters and culture expansion for seeding reactors
Day 2
Lecture:- Application of nuclear transfer to human medicine
- Stem cell research and applications in human regenerative medicine and agriculture
- Immunohistochemistry of stem cell surface markers
- Enucleation and nuclear cell transfer
- Fusion and activation
- Feeder and non-feeder layer setup
- Matrigel coating
- Colony picking and stem cell maintenance
Day 3
Lecture:- Cell Culture-single use bioreactor systems
- Microcarrier and hollow fiber cell culture
- Stirred-tank bioreactor design and operations
- Bench-top reactor setup and preparation for sterilization
- Media handling and asepsis, pH and DO probe preparation and calibration
- Seeding the reactors
Day 4
Lecture:- Cell line characterization for production of biologics
- Rapid detection of mycoplasma in cell culture
- Follow-up on the stirred-tank reactor experiments
Instructors:
- Joseph Camire, M.S., Thermo Fisher Scientific, Inc. USA
- Vikaram Gossain, Ph.D., New Brunswick Scientific Co, Inc. USA
- Kum Park, M.S., Center for Integrated BioSystems, USU
- Kamal Rashid, Ph.D., Center for Integrated BioSystems, USU
- Lee Rickords, Ph.D., Animal, Dairy and Veterinary Sciences, USU
- Tim Taylor, Ph.D., Biological and Irrigation Engineering, USU
- Ken White, Ph.D., Center for Integrated BioSystems, USU