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Graduate Training Courses

Proteomics

Lecture Instructors: Dr. Dong Chen and Dr. Kamal Rashid
Date and Location: June 27, 2006 at the Center for Integrated BioSystems, USU

Course Description

Proteomics is the study of protein pattern for cells, tissues, or organisms at any time point and has become the focal interest of many research areas, including cell biology, biochemistry and medicine. This course will include a brief introduction to the tools used in the proteomics study as well as hands on laboratory work. Students will learn what tools and materials are needed and how to set up their own proteomics study.

Course Agenda

Lectures

  • Introduction to 2-D electrophoresis (Dong Chen)
  • Introduction to protein identification by mass spectrometry (Dong Chen)
  • Introduction to protein expression study by Waters Expression System (Dong Chen)

Laboratory Demo/Exercises

  • Buffer and sample preparation for 2-D electrophoresis (Kum Park)
  • IEF and electrophoresis (Kum Park)
  • Coomassie stain versus Silver Stain (Kum Park)
  • Phoretix 2D gel analysis software (Dong Chen)

Instrumentations

  • BioRad IEF System
  • BioRad Electrophoresis Unit
  • Imagers – Alpha, Kodak and Amersham Typhoon
  • Mass Spectrometer – Waters Q-TOF Premier

Schedule

8:30-10:30am Introduction Lecture
10:30-12:00 Lab: Buffer and sample preparation
BioRad IEF System and first dimensional electrophoresis
12:00-1:00 Lunch (provided)
1:00-2:30 Lab: BioRad Electrophoresis Unit and second dimensional electrophoresis
Coomassie stain versus Silver stain
2:30-3:30 Lab: Phoretix 2D gel analysis software
3:30-4:30 Lab: Mass Spectrometer