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Cell Culture

The Cell Culture facility has two dedicated tissue culture suites equipped with the equipment necessary to maintain and treat a wide range of mammalian and insect cells.

Specialized equipment available includes:

New Brunswick NucleoCounter
  • Zeiss Axiovert 25 Fluorescent Microscope with Digital Camera
  • New Brunswick NucleoCounter
  • Cryomed II Storage Unit

Services available at the Center include:

  1. Flask cell culture and scale up of adherent or suspension cells such as mammalian, hybridoma, and insect cells.
  2. Growth, viability and toxicology studies using small molecules or biocompounds.
  3. Transfection and transformation of cells.
  4. Protein expression and downstream purification.
  5. RNA isolation from cell culture studies.
  6. Fluorescent studies and image capture.

Additionally the CIB has Bioflo 3000 with 5 liter bioreactors available, as well as a Celligen bioreactor cell culture vessel

  1. Cell Lift reactors
  2. Packed Bed reactors
  3. Microcarrier Beads
  4. Sonosep Ultrasonic Separation/Profusion System