Proteomics
Lee Rickords is the Proteomics Group Leader.
The Proteomics Group in the Center for Integrated BioSystems, Utah State University is set up to help researchers in novel biomarker discovery, discovering molecular models for disease, protein identification from complex mixtures with high coverage and global protein expression profiling and protein flux analysis with or without labeling protein mixtures. The CIB Proteomics Group is equipped with gel electrophoresis units, GE Typhoon Laser Imager, Waters nanoACQUITY Ultra Performance LC™ (UPLC™) System and Waters Q-TOF Premier mass spectrometer. We also have software including Nonlinear Phoretix 2D Evolution, Waters ProteinLynx Global SERVER™ (PLGS), Accelrys DS Modeling and Autodock to support high-resolution 2D differential in-gel electrophoresis (DIGE), gel or gel-free and labeling or labeling-free protein expression study, protein modeling and docking study.
Services
Protein Identification
The CIB Proteomics Group offers protein identification by a highly sensitive and accurate mass spectrometer–Waters Q-TOF Premier with Waters nanoACQUITY Ultra Performance LC System. With the nanoLC-ESI-MSMS, we can identify low abundance proteins in a gel or solution. We can also identify many proteins in a protein mixture by Waters IdentityE High Definition Proteomics system. The IdentityE system can combine UPLC/MSE data with a comprehensive peptide ion accounting informatics solution to catalog complex protein digest mixtures.
Intact Protein
Mass Measurement
There are several reasons to analyze intact protein accurate mass. These reasons include confirming protein synthesis, studying the covalent modifications of pure proteins and studying known protein stability. The CIB uses Q-TOF Premier powerful equipment for intact protein mass analysis.
Imaging
The Center offers a variety of platforms that provide imaging capability of visible, UV, phosphorescent, chemiluminescent and fluorescent objects.
Protein Modeling
Some proteins have no 3D structure information.
The CIB Proteomics Group provides protein 3D structure modeling service using Accelrys protein modeling software DS Modeling 1.1.
2-D Difference Gel
Electrophoresis
Services using 2-D difference gel electrophoresis (2D DIGE) are available for a fee in USU's Center for Integrated Biosystems. The 2D DIGE system uses different fluorescent dyes to label control and treated samples, including an internal standard on each gel. This technology effectively minimizes gel-to-gel variation and significantly increases accuracy and reproducibility.
Protein Expression Study
The CIB Proteomics Group offers services that show the protein expression changes between two cell populations (normal vs. cancerous tissue; drug treated vs. untreated, infected vs. uninfected, etc.) by 2D differential in-gel electrophoresis (DIGE) or gel-free and labeling-free Waters Protein Expression System. With the gel-free and labeling-free Waters Protein Expression System, only 50ug of total proteins will be enough for the protein expression study.
Protein-ligand Docking
Protein-ligand docking study is important for new drug discovery. CIB Proteomics Group helps investigators to study protein-ligand docking using leading protein-ligand docking software
– AutoDock.