Affymetrix GeneChip ® System
Sample Requirements
Bioanalyzer
- 50-500 ng of RNA in 1 uL of RNase-free water
- Up to 12 samples can be analyzed at one time.
RNA Labeling
IMPORTANT: The quality of the RNA is essential to the overall success of the analysis.
- A minimum of 5 ug of total RNA at a concentration of >1.1 ug/uL (0.4 ug of mRNA at > 0.4 ug/uL) is required for cDNA synthesis.
- RNA must be pure (devoid of DNA, proteins, etc.) and undegraded. 280/260 ratios of 1.9-2.1 are indications of high quality RNA. Generally, ratios lower than 1.9 indicate potential contamination problems, and ratios higher than 2.1 indicate RNA degradation. Please refer to the Agilent 2100 Bioanalyzer for more information.
- Samples must be in RNase, DNase free 1.5 mL tubes. Please do not submit samples in plates or PCR tubes.
- Please consult with CIB if using prokaryotic RNA.
NOTE: 10 ug or more of total RNA (1 ug of mRNA) is highly recommended. This allows us extra material for the use of quality control and any necessary repeats. If this is not possible, please consult with CIB personnel prior to sample submission.
Hybridization
Please submit labeled samples or hybridization cocktails in RNase, DNase free 1.5 mL tubes.