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454 Next Generation Sequencer

The Genomics core laboratory group provides genomic or high throughput DNA sequencing services using the Roche Genome Sequencer (GS) FLX System (a.k.a. "The 454 Sequencer"). It uses a system based on 454's sequencing by-synthesis technology and features a groundbreaking combination of long reads (of approximately 1 million reads of 400 nt length per full run), exceptional accuracy, and high throughput. Details and uses include:

Services

 General Four-Step Workflow of the GS FLX System:

  • 1) Preparing a DNA library
  • 2) Amplifying the library
  • 3) Sequencing the library
  • 4) Analyzing the data

  • Standard Service:

    Standard Running Cost: ~ $10,000 /per run

    Requirements:
    A minimum of 10µg of high quality DNA (concentration and final volume depending on application) provided by the client. CIB will prepare library, run 454 sequence and perform QC on library preparation and sequencing run. (Cost depending on application and customer requests and agreement).

  • Minimal Service:

    Minimal Running Cost: ~$2,500 to $8,000 /per run

    Requirements:
    High quality library ready for sequence run provided by client. CIB will run 454 sequence and perform QC on sequencing run. (Cost depending on application and customer requests and agreement).

  • Data Handling and Output:

    The sequence data will be available for a limited time on the CIB password protected secure server for client download. Special arrangement (data provided on a DVD disk or on provided HD) are also possible.

  • Data Analysis:

    Data analysis software from Roche is available for customers download. On customer request we can provide assistance on sequence data analysis. The center has a high end data rig and software for most sequence analysis applications as an additional service available for customers.

Next Generation Run Specifications

Next Generation Run Specifications Throughput Read lenght Reads/run 454 Runtime
Full-slide specifications (fragment library run) 400-600MB 400-500bp 1 Million 10 hours
Full-slide specifications (mate-paired library run) 400-600MB 2x175bp 1.2 Million 10 hours

Next Generation Run Specifications 1/2 1/4 1/8 1/16
Partial-slide throughput (fragment library) 230MB 90MB 42MB 8MB
Partial-slide throughput (mate-paired library run) 230MB 90MB 42MB 8MB

Applications

Please review the list of applications below supported by the 454 Sequencer below to find the method best suited for your research.