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Next Generation Sequencing

The Center for Integrated Biosytems offers next generation sequencing services on the Illumina NextSeq, Illumina MiSeq, and Ion Torrent PGM. Applications include whole genome sequencing, RNA-Seq, methylation analysis, amplicon sequencing, genotyping by sequencing (GBS), microRNA sequencing, 16S ribosomal sequencing, ChIP-Seq, targeted sequencing, and more. We also offer size selection on the Blue Pippin automated size selection platform.

NextSeq

Services


Blue Pippin size selection

The Blue Pippin is capable of selecting 100-50,000 bp fragments. However, we do not routinely have the supplies for size selection above 600 bp. Please contact us if you need selection of fragments above 600 bp. Samples are $15 each. Subsequent analysis on the TapeStation is an additional $5 per sample.

 

Library Preparation

We offer standard library preparation for RNA-Seq, microRNA, and whole genome sequencing samples. For other sequencing types, we can assist you with designing your protocol and can work with you as needed to sequence your samples.

DNA library (e.g. whole genome)              $90

RNA-Seq                                                  $110

microRNA                                                 $130

 

Sequencing

While we strive to achieve the best results possible, the listed number of reads is the specification published by Illumina and individual runs may vary from these numbers.

The listed read length can be changed if necessary. Please contact us if you need something different.

 

Illumina MiSeq

 

Internal USU Price

External Price

Expected read number

Typical read length

MiSeq Reagent Kit v3 (150 cycle)

$1,060

$1,500

25M

2X75

MiSeq Reagent Kit v3 (600 cycle)

$1,790

$2,500

25M

2X300

MiSeq Reagent Kit v2 (50 cycle)

$975

$1,370

15M

1x50

MiSeq Reagent Kit v2 (300 cycle)

$1,220

$1,710

15M

2X150

MiSeq Reagent Kit v2 (500 cycle)

$1,350

$1,900

15M

2X250

MiSeq Reagent Micro Kit v2 (300 cycle)

$570

$795

4M

2X150

MiSeq Reagent Nano Kit v2 (300 cycle)

$430

$600

1M

2X150

MiSeq Reagent Nano Kit v2 (500 cycle)

$480

$670

1M

2X250

 

 

Illumina NextSeq

 

Internal USU Price

External Price

Expected read number

Typical read length

NextSeq Mid Output Kit v2 (150 cycles)

$1,255

$1,760

130M

2X75

NextSeq Mid Output Kit v2 (300 cycles)

$1,960

$2,750

130M

2X150

NextSeq High Output Kit v2 (75 cycles)

$1,685

$2,360

400M

1X75

NextSeq High Output Kit v2 (150 cycles)

$3,135

$4,395

400M

2X75

NextSeq High Output Kit v2 (300 cycles)

$4,970

$6,955

400M

2X150

 

 

Ion Torrent PGM

 

Internal USU Price

External Price

Expected read number

Maximum read length

PGM 314

$630

$850

600 K

400

PGM 316

$830

$1120

3 M

400

PGM 318

$1030

$1390

5.5 M

400